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aurumtm total rna mini kit  (Bio-Rad)


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    Bio-Rad aurumtm total rna mini kit
    Aurumtm Total Rna Mini Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 2898 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aurum+total+rna+mini+kits/Aurum+Total+RNA+Mini+Kit/pm42098955-96-32-37
    Average 96 stars, based on 2898 article reviews
    aurumtm total rna mini kit - by Bioz Stars, 2026-09
    96/100 stars

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    Reverse Transcription:

    Article Title: Coronary Microvascular Dysfunction is Associated with Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: Sections were mounted in mounting media and images were taken promptly with the Olympus IX73 imaging system. .. Aurum Total RNA Mini Kits (Bio-Rad, 732-6820) were used to isolate total RNA from heart, aorta, and MCECs. iScript Reverse Transcription Supermix (Bio-Rad, 1708841) was used to generate cDNA from isolated total RNA. .. The Real-Time PCR was performed using the iTaq Universal SYBR Green supermix (Bio-Rad, 1725121) on the Bio-Rad CFX Connect Real-Time System.

    Article Title: Coronary Microvascular Dysfunction Is Associated With Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: DOI: 10.1161/JAHA.124.037460 4 Fisher, A- 31570), Alexa Fluor 555 conjugate anti- rabbit IgG (Thermo Fisher, A- 31572). .. Reagents: Oil red (VWR, BT135140- 100G), Isolectin GS- IB4 (Fisher, I21411), Enzychrom AF cholesterol Assay Kit (BioAssay, E2CH- 100), EnzyChrom Triglyceride Assay Kit (BioAssay, ETGA- 200), bafilomycin A1 from streptomyces griseus (Sigma, B1793), 7- ketocholesterol (Sigma, C2394), Ezetimibe (Cayman, 16331), mitoSOX Red mitochondrial superoxide indicator (moleculae probes M36008), Green FLICA Caspase- 1 Assay Kit (Immunochemistry Technologies, No. 98), Cell- Counting Kit 8 kit (APExBIO; K1018), Aurum Total RNA Mini Kits (Bio- Rad, 732- 6820), iScript Reverse Transcription Supermix for RT- qPCR (BioRad, 1708841), iTaq Universal SYBR Green supermix (Bio- Rad, 1725121). ..

    Article Title: Coronary Microvascular Dysfunction Is Associated With Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: Sections were mounted in mounting media and images were taken promptly with the Olympus IX73 imaging system. .. Aurum Total RNA Mini Kits (Bio‐Rad, 732‐6820) were used to isolate total RNA from heart, aorta, and MCECs. iScript Reverse Transcription Supermix (Bio‐Rad, 1708841) was used to generate cDNA from isolated total RNA. .. Real‐time polymerase chain reaction was performed using the iTaq Universal SYBR Green supermix (Bio‐Rad, 1725121) on the Bio‐Rad CFX Connect Real‐Time System.

    Article Title: Coronary Microvascular Dysfunction Is Associated With Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: Sections were mounted in mounting media and images were taken promptly with the Olympus IX73 imaging system. .. Aurum Total RNA Mini Kits (Bio- Rad, 732- 6820) were used to isolate total RNA from heart, aorta, and MCECs. iScript Reverse Transcription Supermix (Bio- Rad, 1708841) was used to generate cDNA from isolated total RNA. .. Real- time polymerase chain reaction was performed using the iTaq Universal SYBR Green supermix (Bio- Rad, 1725121) on the Bio- Rad CFX Connect Real- Time System.

    Article Title: Cardiovascular dysfunction and altered lysosomal signaling in a murine model of acid sphingomyelinase deficiency.
    Article Snippet: After a final wash with ddH2O, the sections were mounted in mounting media, and images were captured promptly using the Olympus IX73 imaging system. .. Total RNA was isolated from heart tissue using Aurum Total RNA Mini Kits (Bio-Rad, 732–6820). cDNA was synthesized from the isolated RNA using iScript Reverse Transcription Supermix (Bio-Rad, 1,708,841). .. Real-time PCR was conducted with iTaq Universal SYBR Green Supermix (Bio-Rad, 1,725,121) on the Bio-Rad CFX Connect Real-Time System.

    Article Title: Cardiovascular dysfunction and altered lysosomal signaling in a murine model of acid sphingomyelinase deficiency.
    Article Snippet: Secondary antibody for western blot: IRDye® 800 CW anti-Mouse IgG (LICOR 926–32,212), IRDye® 800 CW anti-Rabbit IgG (LICOR 926–32,213), anti-Mouse IgG, HRP (Thermo Fisher A16011), stabilized peroxidase conjugated anti-rabbit (Invitrogen 32,460), and anti-rat IgG-HRP (Fisher 629,520). .. Reagents: Oil red O (VWR BT135140 - 100G), Sirius red (Sigma 365,548 - 5 g), Isolectin GS-IB4 (Fisher I21411), WGA-FITC (Sigma L4895), Aurum Total RNA Mini Kits (Bio-Rad, 732–6820), iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad, 1,708,841), and iTaq Universal SYBR Green Supermix (Bio-Rad, 1,725,121). ..

    Article Title: Coronary Microvascular Dysfunction Is Associated With Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: Secondary antibody for immunofluorescence: anti‐mouse IgG, Alexa Fluor 488 conjugate (Thermo Fisher, A‐21202), Alexa Fluor 488 conjugate anti‐rabbit IgG (Thermo Fisher, A21206), Alexa Fluor 555 conjugate anti‐mouse IgG (Thermo Fisher, A‐31570), Alexa Fluor 555 conjugate anti‐rabbit IgG (Thermo Fisher, A‐31572). .. Reagents: Oil red (VWR, BT135140‐100G), Isolectin GS‐IB4 (Fisher, I21411), Enzychrom AF cholesterol Assay Kit (BioAssay, E2CH‐100), EnzyChrom Triglyceride Assay Kit (BioAssay, ETGA‐200), bafilomycin A1 from streptomyces griseus (Sigma, B1793), 7‐ketocholesterol (Sigma, C2394), Ezetimibe (Cayman, 16331), mitoSOX Red mitochondrial superoxide indicator (moleculae probes M36008), Green FLICA Caspase‐1 Assay Kit (Immunochemistry Technologies, No. 98), Cell‐Counting Kit 8 kit (APExBIO; K1018), Aurum Total RNA Mini Kits (Bio‐Rad, 732‐6820), iScript Reverse Transcription Supermix for RT‐qPCR (Bio‐Rad, 1708841), iTaq Universal SYBR Green supermix (Bio‐Rad, 1725121). ..

    Isolation:

    Article Title: Coronary Microvascular Dysfunction is Associated with Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: Sections were mounted in mounting media and images were taken promptly with the Olympus IX73 imaging system. .. Aurum Total RNA Mini Kits (Bio-Rad, 732-6820) were used to isolate total RNA from heart, aorta, and MCECs. iScript Reverse Transcription Supermix (Bio-Rad, 1708841) was used to generate cDNA from isolated total RNA. .. The Real-Time PCR was performed using the iTaq Universal SYBR Green supermix (Bio-Rad, 1725121) on the Bio-Rad CFX Connect Real-Time System.

    Article Title: Coronary Microvascular Dysfunction Is Associated With Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: Sections were mounted in mounting media and images were taken promptly with the Olympus IX73 imaging system. .. Aurum Total RNA Mini Kits (Bio‐Rad, 732‐6820) were used to isolate total RNA from heart, aorta, and MCECs. iScript Reverse Transcription Supermix (Bio‐Rad, 1708841) was used to generate cDNA from isolated total RNA. .. Real‐time polymerase chain reaction was performed using the iTaq Universal SYBR Green supermix (Bio‐Rad, 1725121) on the Bio‐Rad CFX Connect Real‐Time System.

    Article Title: Coronary Microvascular Dysfunction Is Associated With Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: Sections were mounted in mounting media and images were taken promptly with the Olympus IX73 imaging system. .. Aurum Total RNA Mini Kits (Bio- Rad, 732- 6820) were used to isolate total RNA from heart, aorta, and MCECs. iScript Reverse Transcription Supermix (Bio- Rad, 1708841) was used to generate cDNA from isolated total RNA. .. Real- time polymerase chain reaction was performed using the iTaq Universal SYBR Green supermix (Bio- Rad, 1725121) on the Bio- Rad CFX Connect Real- Time System.

    Article Title: Cardiovascular dysfunction and altered lysosomal signaling in a murine model of acid sphingomyelinase deficiency.
    Article Snippet: After a final wash with ddH2O, the sections were mounted in mounting media, and images were captured promptly using the Olympus IX73 imaging system. .. Total RNA was isolated from heart tissue using Aurum Total RNA Mini Kits (Bio-Rad, 732–6820). cDNA was synthesized from the isolated RNA using iScript Reverse Transcription Supermix (Bio-Rad, 1,708,841). .. Real-time PCR was conducted with iTaq Universal SYBR Green Supermix (Bio-Rad, 1,725,121) on the Bio-Rad CFX Connect Real-Time System.

    Cholesterol Assay:

    Article Title: Coronary Microvascular Dysfunction Is Associated With Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: DOI: 10.1161/JAHA.124.037460 4 Fisher, A- 31570), Alexa Fluor 555 conjugate anti- rabbit IgG (Thermo Fisher, A- 31572). .. Reagents: Oil red (VWR, BT135140- 100G), Isolectin GS- IB4 (Fisher, I21411), Enzychrom AF cholesterol Assay Kit (BioAssay, E2CH- 100), EnzyChrom Triglyceride Assay Kit (BioAssay, ETGA- 200), bafilomycin A1 from streptomyces griseus (Sigma, B1793), 7- ketocholesterol (Sigma, C2394), Ezetimibe (Cayman, 16331), mitoSOX Red mitochondrial superoxide indicator (moleculae probes M36008), Green FLICA Caspase- 1 Assay Kit (Immunochemistry Technologies, No. 98), Cell- Counting Kit 8 kit (APExBIO; K1018), Aurum Total RNA Mini Kits (Bio- Rad, 732- 6820), iScript Reverse Transcription Supermix for RT- qPCR (BioRad, 1708841), iTaq Universal SYBR Green supermix (Bio- Rad, 1725121). ..

    Article Title: Coronary Microvascular Dysfunction is Associated with Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: Secondary antibody for immunofluorescence: anti-Mouse IgG, Alexa Fluor 488 conjugate (Thermo Fisher A-21202), Alexa Fluor 488 conjugate anti-Rabbit IgG (Thermo Fisher A21206), Alexa Fluor 555 conjugate anti-Mouse IgG (Thermo Fisher A-31570), Alexa Fluor 555 conjugate anti-Rabbit IgG (Thermo Fisher A-31572). .. Reagents: Oil red (VWR BT135140-100G), Isolectin GS-IB4 (Fisher I21411), Enzychrom AF cholesterol Assay Kit (BioAssay E2CH-100), EnzyChrom Triglyceride Assay Kit (BioAssay ETGA-200), Bafilomycin A1 from streptomyces griseus (Sigma B1793), 7-Ketocholesterol (7-keto, Sigma C2394), Ezetimibe (Cayman 16331), mitoSOX® Red mitochondrial superoxide indicator (moleculae probes M36008), Green FLICA Caspase-1 Assay Kit (Immunochemistry Technologies, #98), CCK8 kit (APExBIO, K1018), Aurum Total RNA Mini Kits (Bio-Rad, 732-6820), iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad, 1708841), iTaq Universal SYBR Green supermix (Bio-Rad, 1725121). ..

    Article Title: Coronary Microvascular Dysfunction Is Associated With Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: Secondary antibody for immunofluorescence: anti‐mouse IgG, Alexa Fluor 488 conjugate (Thermo Fisher, A‐21202), Alexa Fluor 488 conjugate anti‐rabbit IgG (Thermo Fisher, A21206), Alexa Fluor 555 conjugate anti‐mouse IgG (Thermo Fisher, A‐31570), Alexa Fluor 555 conjugate anti‐rabbit IgG (Thermo Fisher, A‐31572). .. Reagents: Oil red (VWR, BT135140‐100G), Isolectin GS‐IB4 (Fisher, I21411), Enzychrom AF cholesterol Assay Kit (BioAssay, E2CH‐100), EnzyChrom Triglyceride Assay Kit (BioAssay, ETGA‐200), bafilomycin A1 from streptomyces griseus (Sigma, B1793), 7‐ketocholesterol (Sigma, C2394), Ezetimibe (Cayman, 16331), mitoSOX Red mitochondrial superoxide indicator (moleculae probes M36008), Green FLICA Caspase‐1 Assay Kit (Immunochemistry Technologies, No. 98), Cell‐Counting Kit 8 kit (APExBIO; K1018), Aurum Total RNA Mini Kits (Bio‐Rad, 732‐6820), iScript Reverse Transcription Supermix for RT‐qPCR (Bio‐Rad, 1708841), iTaq Universal SYBR Green supermix (Bio‐Rad, 1725121). ..

    Bioassay:

    Article Title: Coronary Microvascular Dysfunction Is Associated With Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: DOI: 10.1161/JAHA.124.037460 4 Fisher, A- 31570), Alexa Fluor 555 conjugate anti- rabbit IgG (Thermo Fisher, A- 31572). .. Reagents: Oil red (VWR, BT135140- 100G), Isolectin GS- IB4 (Fisher, I21411), Enzychrom AF cholesterol Assay Kit (BioAssay, E2CH- 100), EnzyChrom Triglyceride Assay Kit (BioAssay, ETGA- 200), bafilomycin A1 from streptomyces griseus (Sigma, B1793), 7- ketocholesterol (Sigma, C2394), Ezetimibe (Cayman, 16331), mitoSOX Red mitochondrial superoxide indicator (moleculae probes M36008), Green FLICA Caspase- 1 Assay Kit (Immunochemistry Technologies, No. 98), Cell- Counting Kit 8 kit (APExBIO; K1018), Aurum Total RNA Mini Kits (Bio- Rad, 732- 6820), iScript Reverse Transcription Supermix for RT- qPCR (BioRad, 1708841), iTaq Universal SYBR Green supermix (Bio- Rad, 1725121). ..

    Article Title: Coronary Microvascular Dysfunction is Associated with Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: Secondary antibody for immunofluorescence: anti-Mouse IgG, Alexa Fluor 488 conjugate (Thermo Fisher A-21202), Alexa Fluor 488 conjugate anti-Rabbit IgG (Thermo Fisher A21206), Alexa Fluor 555 conjugate anti-Mouse IgG (Thermo Fisher A-31570), Alexa Fluor 555 conjugate anti-Rabbit IgG (Thermo Fisher A-31572). .. Reagents: Oil red (VWR BT135140-100G), Isolectin GS-IB4 (Fisher I21411), Enzychrom AF cholesterol Assay Kit (BioAssay E2CH-100), EnzyChrom Triglyceride Assay Kit (BioAssay ETGA-200), Bafilomycin A1 from streptomyces griseus (Sigma B1793), 7-Ketocholesterol (7-keto, Sigma C2394), Ezetimibe (Cayman 16331), mitoSOX® Red mitochondrial superoxide indicator (moleculae probes M36008), Green FLICA Caspase-1 Assay Kit (Immunochemistry Technologies, #98), CCK8 kit (APExBIO, K1018), Aurum Total RNA Mini Kits (Bio-Rad, 732-6820), iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad, 1708841), iTaq Universal SYBR Green supermix (Bio-Rad, 1725121). ..

    Article Title: Coronary Microvascular Dysfunction Is Associated With Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: Secondary antibody for immunofluorescence: anti‐mouse IgG, Alexa Fluor 488 conjugate (Thermo Fisher, A‐21202), Alexa Fluor 488 conjugate anti‐rabbit IgG (Thermo Fisher, A21206), Alexa Fluor 555 conjugate anti‐mouse IgG (Thermo Fisher, A‐31570), Alexa Fluor 555 conjugate anti‐rabbit IgG (Thermo Fisher, A‐31572). .. Reagents: Oil red (VWR, BT135140‐100G), Isolectin GS‐IB4 (Fisher, I21411), Enzychrom AF cholesterol Assay Kit (BioAssay, E2CH‐100), EnzyChrom Triglyceride Assay Kit (BioAssay, ETGA‐200), bafilomycin A1 from streptomyces griseus (Sigma, B1793), 7‐ketocholesterol (Sigma, C2394), Ezetimibe (Cayman, 16331), mitoSOX Red mitochondrial superoxide indicator (moleculae probes M36008), Green FLICA Caspase‐1 Assay Kit (Immunochemistry Technologies, No. 98), Cell‐Counting Kit 8 kit (APExBIO; K1018), Aurum Total RNA Mini Kits (Bio‐Rad, 732‐6820), iScript Reverse Transcription Supermix for RT‐qPCR (Bio‐Rad, 1708841), iTaq Universal SYBR Green supermix (Bio‐Rad, 1725121). ..

    Cell Counting:

    Article Title: Coronary Microvascular Dysfunction Is Associated With Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: DOI: 10.1161/JAHA.124.037460 4 Fisher, A- 31570), Alexa Fluor 555 conjugate anti- rabbit IgG (Thermo Fisher, A- 31572). .. Reagents: Oil red (VWR, BT135140- 100G), Isolectin GS- IB4 (Fisher, I21411), Enzychrom AF cholesterol Assay Kit (BioAssay, E2CH- 100), EnzyChrom Triglyceride Assay Kit (BioAssay, ETGA- 200), bafilomycin A1 from streptomyces griseus (Sigma, B1793), 7- ketocholesterol (Sigma, C2394), Ezetimibe (Cayman, 16331), mitoSOX Red mitochondrial superoxide indicator (moleculae probes M36008), Green FLICA Caspase- 1 Assay Kit (Immunochemistry Technologies, No. 98), Cell- Counting Kit 8 kit (APExBIO; K1018), Aurum Total RNA Mini Kits (Bio- Rad, 732- 6820), iScript Reverse Transcription Supermix for RT- qPCR (BioRad, 1708841), iTaq Universal SYBR Green supermix (Bio- Rad, 1725121). ..

    Article Title: Coronary Microvascular Dysfunction Is Associated With Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: Secondary antibody for immunofluorescence: anti‐mouse IgG, Alexa Fluor 488 conjugate (Thermo Fisher, A‐21202), Alexa Fluor 488 conjugate anti‐rabbit IgG (Thermo Fisher, A21206), Alexa Fluor 555 conjugate anti‐mouse IgG (Thermo Fisher, A‐31570), Alexa Fluor 555 conjugate anti‐rabbit IgG (Thermo Fisher, A‐31572). .. Reagents: Oil red (VWR, BT135140‐100G), Isolectin GS‐IB4 (Fisher, I21411), Enzychrom AF cholesterol Assay Kit (BioAssay, E2CH‐100), EnzyChrom Triglyceride Assay Kit (BioAssay, ETGA‐200), bafilomycin A1 from streptomyces griseus (Sigma, B1793), 7‐ketocholesterol (Sigma, C2394), Ezetimibe (Cayman, 16331), mitoSOX Red mitochondrial superoxide indicator (moleculae probes M36008), Green FLICA Caspase‐1 Assay Kit (Immunochemistry Technologies, No. 98), Cell‐Counting Kit 8 kit (APExBIO; K1018), Aurum Total RNA Mini Kits (Bio‐Rad, 732‐6820), iScript Reverse Transcription Supermix for RT‐qPCR (Bio‐Rad, 1708841), iTaq Universal SYBR Green supermix (Bio‐Rad, 1725121). ..

    Quantitative RT-PCR:

    Article Title: Coronary Microvascular Dysfunction Is Associated With Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: DOI: 10.1161/JAHA.124.037460 4 Fisher, A- 31570), Alexa Fluor 555 conjugate anti- rabbit IgG (Thermo Fisher, A- 31572). .. Reagents: Oil red (VWR, BT135140- 100G), Isolectin GS- IB4 (Fisher, I21411), Enzychrom AF cholesterol Assay Kit (BioAssay, E2CH- 100), EnzyChrom Triglyceride Assay Kit (BioAssay, ETGA- 200), bafilomycin A1 from streptomyces griseus (Sigma, B1793), 7- ketocholesterol (Sigma, C2394), Ezetimibe (Cayman, 16331), mitoSOX Red mitochondrial superoxide indicator (moleculae probes M36008), Green FLICA Caspase- 1 Assay Kit (Immunochemistry Technologies, No. 98), Cell- Counting Kit 8 kit (APExBIO; K1018), Aurum Total RNA Mini Kits (Bio- Rad, 732- 6820), iScript Reverse Transcription Supermix for RT- qPCR (BioRad, 1708841), iTaq Universal SYBR Green supermix (Bio- Rad, 1725121). ..

    Article Title: Coronary Microvascular Dysfunction is Associated with Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: Secondary antibody for immunofluorescence: anti-Mouse IgG, Alexa Fluor 488 conjugate (Thermo Fisher A-21202), Alexa Fluor 488 conjugate anti-Rabbit IgG (Thermo Fisher A21206), Alexa Fluor 555 conjugate anti-Mouse IgG (Thermo Fisher A-31570), Alexa Fluor 555 conjugate anti-Rabbit IgG (Thermo Fisher A-31572). .. Reagents: Oil red (VWR BT135140-100G), Isolectin GS-IB4 (Fisher I21411), Enzychrom AF cholesterol Assay Kit (BioAssay E2CH-100), EnzyChrom Triglyceride Assay Kit (BioAssay ETGA-200), Bafilomycin A1 from streptomyces griseus (Sigma B1793), 7-Ketocholesterol (7-keto, Sigma C2394), Ezetimibe (Cayman 16331), mitoSOX® Red mitochondrial superoxide indicator (moleculae probes M36008), Green FLICA Caspase-1 Assay Kit (Immunochemistry Technologies, #98), CCK8 kit (APExBIO, K1018), Aurum Total RNA Mini Kits (Bio-Rad, 732-6820), iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad, 1708841), iTaq Universal SYBR Green supermix (Bio-Rad, 1725121). ..

    Article Title: Cardiovascular dysfunction and altered lysosomal signaling in a murine model of acid sphingomyelinase deficiency.
    Article Snippet: Secondary antibody for western blot: IRDye® 800 CW anti-Mouse IgG (LICOR 926–32,212), IRDye® 800 CW anti-Rabbit IgG (LICOR 926–32,213), anti-Mouse IgG, HRP (Thermo Fisher A16011), stabilized peroxidase conjugated anti-rabbit (Invitrogen 32,460), and anti-rat IgG-HRP (Fisher 629,520). .. Reagents: Oil red O (VWR BT135140 - 100G), Sirius red (Sigma 365,548 - 5 g), Isolectin GS-IB4 (Fisher I21411), WGA-FITC (Sigma L4895), Aurum Total RNA Mini Kits (Bio-Rad, 732–6820), iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad, 1,708,841), and iTaq Universal SYBR Green Supermix (Bio-Rad, 1,725,121). ..

    SYBR Green Assay:

    Article Title: Coronary Microvascular Dysfunction Is Associated With Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: DOI: 10.1161/JAHA.124.037460 4 Fisher, A- 31570), Alexa Fluor 555 conjugate anti- rabbit IgG (Thermo Fisher, A- 31572). .. Reagents: Oil red (VWR, BT135140- 100G), Isolectin GS- IB4 (Fisher, I21411), Enzychrom AF cholesterol Assay Kit (BioAssay, E2CH- 100), EnzyChrom Triglyceride Assay Kit (BioAssay, ETGA- 200), bafilomycin A1 from streptomyces griseus (Sigma, B1793), 7- ketocholesterol (Sigma, C2394), Ezetimibe (Cayman, 16331), mitoSOX Red mitochondrial superoxide indicator (moleculae probes M36008), Green FLICA Caspase- 1 Assay Kit (Immunochemistry Technologies, No. 98), Cell- Counting Kit 8 kit (APExBIO; K1018), Aurum Total RNA Mini Kits (Bio- Rad, 732- 6820), iScript Reverse Transcription Supermix for RT- qPCR (BioRad, 1708841), iTaq Universal SYBR Green supermix (Bio- Rad, 1725121). ..

    Article Title: Coronary Microvascular Dysfunction is Associated with Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: Secondary antibody for immunofluorescence: anti-Mouse IgG, Alexa Fluor 488 conjugate (Thermo Fisher A-21202), Alexa Fluor 488 conjugate anti-Rabbit IgG (Thermo Fisher A21206), Alexa Fluor 555 conjugate anti-Mouse IgG (Thermo Fisher A-31570), Alexa Fluor 555 conjugate anti-Rabbit IgG (Thermo Fisher A-31572). .. Reagents: Oil red (VWR BT135140-100G), Isolectin GS-IB4 (Fisher I21411), Enzychrom AF cholesterol Assay Kit (BioAssay E2CH-100), EnzyChrom Triglyceride Assay Kit (BioAssay ETGA-200), Bafilomycin A1 from streptomyces griseus (Sigma B1793), 7-Ketocholesterol (7-keto, Sigma C2394), Ezetimibe (Cayman 16331), mitoSOX® Red mitochondrial superoxide indicator (moleculae probes M36008), Green FLICA Caspase-1 Assay Kit (Immunochemistry Technologies, #98), CCK8 kit (APExBIO, K1018), Aurum Total RNA Mini Kits (Bio-Rad, 732-6820), iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad, 1708841), iTaq Universal SYBR Green supermix (Bio-Rad, 1725121). ..

    Article Title: Cardiovascular dysfunction and altered lysosomal signaling in a murine model of acid sphingomyelinase deficiency.
    Article Snippet: Secondary antibody for western blot: IRDye® 800 CW anti-Mouse IgG (LICOR 926–32,212), IRDye® 800 CW anti-Rabbit IgG (LICOR 926–32,213), anti-Mouse IgG, HRP (Thermo Fisher A16011), stabilized peroxidase conjugated anti-rabbit (Invitrogen 32,460), and anti-rat IgG-HRP (Fisher 629,520). .. Reagents: Oil red O (VWR BT135140 - 100G), Sirius red (Sigma 365,548 - 5 g), Isolectin GS-IB4 (Fisher I21411), WGA-FITC (Sigma L4895), Aurum Total RNA Mini Kits (Bio-Rad, 732–6820), iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad, 1,708,841), and iTaq Universal SYBR Green Supermix (Bio-Rad, 1,725,121). ..

    Article Title: Coronary Microvascular Dysfunction Is Associated With Augmented Lysosomal Signaling in Hypercholesterolemic Mice
    Article Snippet: Secondary antibody for immunofluorescence: anti‐mouse IgG, Alexa Fluor 488 conjugate (Thermo Fisher, A‐21202), Alexa Fluor 488 conjugate anti‐rabbit IgG (Thermo Fisher, A21206), Alexa Fluor 555 conjugate anti‐mouse IgG (Thermo Fisher, A‐31570), Alexa Fluor 555 conjugate anti‐rabbit IgG (Thermo Fisher, A‐31572). .. Reagents: Oil red (VWR, BT135140‐100G), Isolectin GS‐IB4 (Fisher, I21411), Enzychrom AF cholesterol Assay Kit (BioAssay, E2CH‐100), EnzyChrom Triglyceride Assay Kit (BioAssay, ETGA‐200), bafilomycin A1 from streptomyces griseus (Sigma, B1793), 7‐ketocholesterol (Sigma, C2394), Ezetimibe (Cayman, 16331), mitoSOX Red mitochondrial superoxide indicator (moleculae probes M36008), Green FLICA Caspase‐1 Assay Kit (Immunochemistry Technologies, No. 98), Cell‐Counting Kit 8 kit (APExBIO; K1018), Aurum Total RNA Mini Kits (Bio‐Rad, 732‐6820), iScript Reverse Transcription Supermix for RT‐qPCR (Bio‐Rad, 1708841), iTaq Universal SYBR Green supermix (Bio‐Rad, 1725121). ..

    Synthesized:

    Article Title: Cardiovascular dysfunction and altered lysosomal signaling in a murine model of acid sphingomyelinase deficiency.
    Article Snippet: After a final wash with ddH2O, the sections were mounted in mounting media, and images were captured promptly using the Olympus IX73 imaging system. .. Total RNA was isolated from heart tissue using Aurum Total RNA Mini Kits (Bio-Rad, 732–6820). cDNA was synthesized from the isolated RNA using iScript Reverse Transcription Supermix (Bio-Rad, 1,708,841). .. Real-time PCR was conducted with iTaq Universal SYBR Green Supermix (Bio-Rad, 1,725,121) on the Bio-Rad CFX Connect Real-Time System.

    Whole Genome Amplification:

    Article Title: Cardiovascular dysfunction and altered lysosomal signaling in a murine model of acid sphingomyelinase deficiency.
    Article Snippet: Secondary antibody for western blot: IRDye® 800 CW anti-Mouse IgG (LICOR 926–32,212), IRDye® 800 CW anti-Rabbit IgG (LICOR 926–32,213), anti-Mouse IgG, HRP (Thermo Fisher A16011), stabilized peroxidase conjugated anti-rabbit (Invitrogen 32,460), and anti-rat IgG-HRP (Fisher 629,520). .. Reagents: Oil red O (VWR BT135140 - 100G), Sirius red (Sigma 365,548 - 5 g), Isolectin GS-IB4 (Fisher I21411), WGA-FITC (Sigma L4895), Aurum Total RNA Mini Kits (Bio-Rad, 732–6820), iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad, 1,708,841), and iTaq Universal SYBR Green Supermix (Bio-Rad, 1,725,121). ..



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    aurum rna mini isolation kit - by Bioz Stars, 2026-09
    96/100 stars
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